Melyta Rizky Pratami
Department of Chemical Engineering, University of Jember, Indonesia 68121

Rizki Fitria Darmayanti
Department of Chemical Engineering, University of Jember, Indonesia 68121

Nanda Febia Nuraini
Department of Chemical Engineering, University of Jember, Indonesia 68121

Faizzatul Munawaroh

Department of Chemical Engineering, University of Jember, Indonesia 68121

DOI:https://doi.org/10.19184/jobc.v2i1.116      

ABSTRACT
Reducing sugar can be produced from lignocellulosic raw materials. The content of polysaccharides such as cellulose, hemicellulose, and starch will be broken down into simpler carbohydrates. This study used a mixture of sugarcane bagasse and rice husks as lignocellulosic raw materials. The lignin content in the raw material must be removed through delignification or pretreatment so that enzymes can access cellulose and hemicellulose. This study used a physicochemical pretreatment method, in which lignocellulosic material was soaked in 3% NaOH, then heated with microwave and boiling water. The following process is enzymatic hydrolysis with variations of cellulase enzyme activity 0.434, 0.871, 2.61, and 3.49 FPU/g mixture of bagasse and rice husks. The cellulase enzyme used in this study was also derived from the fungus Trichoderma viride. Analysis of the sugar concentration resulting from hydrolysis used the DNS method with the 3.5-dinitrosalicylic acid reagent. The concentration of sugar from hydrolysis using a variety of enzymes with microwave heating pretreatment and boiling water pretreatment obtained the highest results, which were the same at the addition of enzyme activity 3.49 FPU/g substrate at 24 hours, namely 4.077 g/L and 15.18 g/L. The optimum time for enzymatic hydrolysis is 12 hours, and the optimum enzyme activity is the addition of 2.61 FPU/g. The average concentration of sugar hydrolyzed by the addition of Trichoderma viride solution in pretreatment using microwave heating was 0.7611 g/L, with a yield of 21.01 mg sugar/g substrate, and with pretreatment in boiling water, it was 0.8679 g/L, with a yield of 23.95 mg sugar/g substrate.

Keywords: sugarcane bagasse, rice husk, enzymatic hydrolysis, lignocellulose, reducing sugar, and Trichoderma viride

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