Extraction of Bioactive Compounds from Coffee Husk with Acetone using Microwave Assisted Extraction Method and Analysis of Phenolic Compound
Extraction of Bioactive Compounds from Coffee Husk with Acetone using Microwave Assisted Extraction Method and Analysis of Phenolic Compound
Theressa Julieta Putri Andina
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Helda Wika Amini
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Ansori Ansori
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Yukti Nurani
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Ratri Sekaringgalih
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Sonya Hakim Raharjo
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
Merymistika Yufrani Afred
Department of Chemical Engineering, Faculty of Engineering, Jember University, Indonesia
DOI: https://doi.org/10.19184/jobc.v4i2.1357
ABSTRACT
Nowadays, coffee husks are limited to animal feed and fertilizer. Therefore, a study used Robusta coffee husk waste as a raw material. The purpose of this study was to determine the effect of several variables and to determine the optimum conditions in the process of extracting bioactive compounds from coffee husk waste. The coffee husk contains bioactive compounds, including anthocyanins and polyphenol compounds, such as flavonols, flavan-3-ols, hydroxycinnamic acids, and caffeine. Coffee husk waste will be extracted using the Microwave-Assisted Extraction (MAE) method with acetone solvent, with several variables: the ratio of material to solvent, microwave power, and extraction time. Analysis of the identification of phenolic compound content was then also carried out using UV-Vis spectrophotometry. The variables of material ratio, extraction time, and microwave power were proven to be interrelated so that they could produce total phenol at optimum conditions. The optimum conditions for extracting bioactive compounds from coffee husk waste were obtained at a material ratio of 0.04 g/mL, 9 minutes, and a power of 300 watts, with a total phenol yield of 8.65 GAE/g sample.
Keywords: coffee husk, acetone, extraction, MAE, total phenol
REFERENCES
[1] R. Agustin, D. Novita, H. Pratama, S. Sela, & S. Chintya. (2020). Analisis Korelasi Luas Lahan Serta Ekspor Kopi Lampung Terhadap Ekspor Kopi Indonesia. Indones. J. Appl. Math., vol. 1, no. 1, pp. 25–30.
[2] H. Prasetyo. (2015). Ekstraksi Senyawa Antioksidan Kulit Buah Kopi: Kajian Jenis Kopi dan Lama Maserasi. Skripsi.
[3] A. I. Juwita, A. Mustafa, & R. Tamrin. (2017). Studi Pemanfaatan Kulit Kopi Arabika (Coffee arabica L.) Sebagai Mikro Organisme Lokal (MOL). Agrointek, vol. 11, no. 1, p. 1. doi: 10.21107/agrointek.v11i1.2937.
[4] G. Munguía-Ameca, M. E. Ortega-Cerrilla, P. Zetina.Córdoba, A. D. , J. Herrera-Haro, R. Guinzberg-Perrusquía, & R. B.-G., M. Soto-Hernández. (2015). Chemical Composition, Antioxidant Compounds and Antioxidant Capacity of Ensiled Coffee Pulp. 6th Int. Semin. Trop. Anim. Prod., pp. 177–181.
[5] C. E. Dhurhania & A. Novianto. (2019). Uji Kandungan Fenolik Total dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens). J. Farm. Dan Ilmu Kefarmasian Indones., vol. 5, no. 2, p. 62. doi: 10.20473/jfiki.v5i22018.62-68.
[6] Febriyanto, N. I. Hanifa, & H. Muliasari. (2021). Penetapan Kadar Fenolik Total Ekstrak Kulit Buah Kopi Robusta (Coffea canephora L.) di Pulau Lombok. Lumbung Farm. J. Ilmu Kefarmasian, vol. 2, no. 2, p. 89. doi: 10.31764/lf.v2i2.5489.
[7] Y. A. T. Wahyuni, G. A. Kadek Diah Puspawati, & I. N. Kencana Putra. (2021). Pengaruh Jenis Pelarut pada Metode Microwave Assisted Extraction (MAE) terhadap Karakteristik Ekstrak Daun Singkong (Manihot utilissima Pohl.). J. Ilmu dan Teknol. Pangan, vol. 10, no. 4, p. 566. doi: 10.24843/itepa.2021.v10.i04.p03.
[8] A. Fahmi Arwangga, I. A. Raka Astiti Asih, & I. W. Sudiarta. (2016). Analisis Kandungan Kafein Pada Kopi di Desa Sesaot Narmada Menggunakan Spektrofotometri Uv-Vis. J. Kim., vol. 10, no. 1, pp. 110–114. doi: 10.24843/jchem.2016.v10.i01.p15.
[9] B. L. Sari, T. Triastinurmiatiningsih, T. S. Haryani. (2020). Optimasi Metode Microwave-Assisted Extraction(MAE) untuk Menentukan Kadar Flavonoid Total Alga Coklat Padina australis. ALCHEMY J. Penelit. Kim., vol. 16, no. 1, p. 38. doi: 10.20961/alchemy.16.1.34186.38-49.
[10] N. Ayuchecaria, M. M. Alfiannor Saputera, & R. Niah. (2020). Penentapan Kadar Fenolik Total Ekstrak Batang Bajakah Tampala (Spatholobus littoralis Hassk.) Menggunakan Spektrofotometri Uv-Visible. J. Insa. Farm. Indones., vol. 3, no. 1, pp. 132–141. doi: 10.36387/jifi.v3i1.478.
[11] R. N. Indah, S. P. S. Saraswati. (2021). Penyerapan Logam Magnesium dengan Menggunakan Bubuk Alga Merah (Gracilaria Sp). J. Tek. Kim., vol. 15, no. 2, pp. 0–4. doi: 10.33005/jurnal_tekkim.v15i2.2542.
[12] N. I. Karmila. Defi, Dwi Cahyono. Tomy. (2022). Effect of Variation of Ginger Extract On Color Brightness, Water Content, Degree of Acidity (pH) and Organoleptic Quality of Jelly Candy Cashew Extract (Anacardium occidentale L). 3, pp. 55–72,.
[13] K. A. Septiani, N. O. A. Parwata, & I. A. A. B. Putra. (2018). Penentuan Kadar Total Fenol, Kadar Total Flavonoid dan Skrining Fitokimia Ekstrak Etanol Daun Gaharu (Gyrinops versteegii). J. Mat., vol. 12, no. 1, pp. 78–89.
[14] P. K. Naraswanik. (2021). Isolasi dan Identifikasi Senyawa Flavonoid Daun Kelor (Moringa oleofera L.) dengan Metode Ekstraksi Ultrasonik. Skripsi.
[15] T. Arikalang, S. Sudewi, & J. Rorong. (2018). Optimasi dan Validasi Metode Analisis dalam Penentuan Kandungan Total Fenolik pada Ekstrak Daun Gedi Hijau (Abelmoschus manihot L.) yang Diukur dengan Spektrofotometri UV-Vis. J. Ilm. Farm., vol. 7, no. 3, pp. 14–21.
[16] S. A. Awal. (2022). Optimasi Parameter Ekstraksi Secara Microwave Assisted Extraction untuk Memperoleh Viteksikarpin Pada Daun Vitex trifolia Linn. Skripsi.
[17] G. U. S. P. Rahayu, G. P. G. Putra, & L. P. Wrasiati. (2022). Pengaruh Rasio Bahan:Pelarut dan Waktu Ekstraksi dengan Gelombang Mikro terhadap Ekstrak Etanol Kulit Buah Kopi Robusta Sebagai Sumber Antioksidan. J. Rekayasa Dan Manaj. Agroindustri, vol. 10, no. 4, pp. 388–397. doi: 10.24843/jrma.2022.v10.i04.p01.
[18] A. Faadhilah. (2019). Optimasi Microwave Assisted Extraction Terhadap Senyawa Bioaktif Antioksidan dari Sarang Semut Papua (Myrmecodia Pendans) dengan Variasi Konsentrasi Etanol, Suhu dan Lama Ekstraksi. J. Chem. Inf. Model., vol. 53, no. 9, pp. 1689–1699.
[19] I. S. M. Purbowati et al. (2021). Pengaruh Metode dan Variasi Waktu Ekstraksi terhadap Total Fenol Ekstrak Daun Sereh Wangi (Cymbopogon nardus L). Pros. Semin. Nas. dan Call Pap., pp. 202–206.
[20] B. Yingngam, A. Chiangsom, & A. Brantner. (2020). Modeling and Optimization of Microwave-Assisted Extraction of Pentacyclic Triterpenes from Centella Asiatica Leaves Using Response Surface Methodology. Ind. Crops Prod., vol. 147, no. October 2019, p. 112231. doi: 10.1016/j.indcrop.2020.1122
Published
05-12-2024
Issue
Vol. 4 Issue 2 (2024): JOBC: Journal of Biobased Chemicals
Pages
126-139
License
Copyright (c) 2024 JOBC: Journal of Biobased Chemicals
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