Slamet Pujianto
Department of Chemical Engineering, University of Jember, Indonesia 68121

Boy Arief Fachri
Department of Chemical Engineering, University of Jember, Indonesia 68121

Erna Subroto
Crop Protection Pte, Ltd, Singapore

Istiqomah Rahmawati
Department of Chemical Engineering, University of Jember, Indonesia 68121



DOI:https://doi.org/10.19184/jobc.v2i2.271             

ABSTRACT
Sargassum sp., part of brown algae, is traditionally used as animal feed. Nonetheless, Sargassum contains phenolic compounds that promise to be the raw material of natural antioxidants. The work is to extract phenolic compounds from Sargassum sp. in ethanol and to investigate the effect of the process on phenol yields. Several dried Sargassum sp. are milled in a certain size and extraction using ultrasound assisted extraction with ethanol solvent. Process variables are the extraction time (30-50 minutes) and the Ratio of Sargassum sp. to ethanol which is 5:100-15:100 (b/v), and power of 170-190 watts. Phenol compounds are quantified using the error acid method. The DPPH method is performed to check the antioxidant activity. To investigate the influence of the process, surface response methods based on central composite designs are applied in this work. 153.334 mgGAE/g in 30-minute extraction conditions, 170 watts of power, and a ratio between masses and solvents of 0.05. The antioxidant activity (IC50) of Sargassum sp. extract is 87.57 ppm.

Keywords: Sargassum sp., phenolic compound, ethanol, ultrasound assisted extraction

REFERENCES

[1] Erniati, Zakaria FR, Prangdimurti E, Adawiyah DR. 2016. Seaweed potential: bioactive compounds studies and its utilization as a functional food product. Aquatic Sciences Journal. 3(1): 12-17.

[2] Mekini ́c IG, Skroza D, Šimat V, Hamed I, Cagalj M, Perkovi ́c ZP. 2019. Phenolic content of brown algae (Pheophyceae) species: extraction, identification, and quantification. Biomolecules. 9(6):244

[3] Rohim, A., Yunianta., & Teti Estiasih. 2019. Senyawa-senyawa Biokatif Pada Rumput Laut Cokelat Sargassum Sp. : Ulasan Ilmiah. Jurnal Teknologi Pertanian.Universitas Brawijaya. Vol.20. No.2

[4] Abdullah A, Nurjanah, Nasution AIS. 2021. Karakteristik fraksi aktif biopigmen fukosantin rumput laut cokelat sebagai antioksidan dan UV-protector. Jurnal Pengolahan Hasil Perikanan Indonesia. 24(1): 131-147.

[5] Maharany F, Nurjanah, Suwandi R, Anwar E, Hidayat T. 2017. Kandungan senyawa bioaktif rumput laut Padina australis dan Eucheuma cottonii sebagai bahan baku krim tabir surya. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(1): 11-18.

[6] Firdaus,Muhammad.2019. Pigmen Rumput Laut dan Manfaat Kesehatannya. Malang.UB Press. 

[7] Khairunnisa, Karina , Efri Mardawati, and Harnesa Selly Putri. 2020. Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Propolis Lebah Trigona Sp. JURNAL INDUSTRI PERTANIAN. 2(1): 124-129

[8] Sekarsari, S., Widarta, I.W.R., dan Jambe, A.G.D.N.A. 2019. Pengaruh Suhu dan Waktu Ekstraksi dengan Gelombang Ultrasonik Terhadap Aktivitas Antioksidan Ekstrak Daun Jambu Biji (Psidium guajav L.). Jurnal Ilmu dan Teknologi Pangan, 8(3):267-277.