Fira Ulvatur Rohmah
MAN 3 Jembrana, Indonesia

Rina Auliyah Safitri
PT Vemci Kosmetik Banyuwangi, Indonesia

Ayu Ulum Rahmawati
PT Mitra Alam Segar Pasuruan, Indonesia

Atiqa Rahmawati
Department of Leather Engineering and Skin Preservation, Politeknik ATK Yogyakarta, Indonesia

DOI: https://doi.org/10.19184/jobc.v2i2.274          

ABSTRACT
The Pegagan plant has a scientific name in the form of Centella asiatica L., which is included in the Centella genus, Apiaceae family, and kingdom Plantae. Pegagan (Centella asiatica L.) has distinctive bioactive components, namely triterpene ester glycoside compounds in the form of asiaticoside and madecassoside, and triterpene group compounds in the form of Asiatic acid and madecassic acid. In this study, the extraction of bioactive components from pegagan was carried out using the Microwave-Assisted Extraction (MAE) method, and the effect of ethanol solvent concentration on the extraction of bioactive compounds was studied. Analysis of the extracted bioactive content was carried out by analyzing the total phenol content using the Folin-Ciocalteau reagent and ANOVA analysis. The results obtained from the study were in the form of total phenol content as an indication of the presence of bioactive compounds, namely at operating conditions of 450 watts of power, 50% ethanol concentration, with a radiation time of 15 minutes, which resulted in a total phenol content of 21.9244 mg AGE/g sample. In the ANOVA analysis with ethanol solvent, variables that gave a significant response to the total phenol content were microwave power, radiation time, and ethanol concentration, with an R-square value of 95.31%. The effect of ethanol concentration on the total phenol content produced, namely the concentration of pure ethanol solvent, will produce extracts with the smallest total phenol content. The effect of extraction time on total phenol content, namely, the longer the extraction time, will increase the total phenol content. Maximum total phenol content using ethanol solvent, that is, at operating conditions of 450 watts of power, 10% ethanol concentration, with a radiation time of 15 minutes, which resulted in a total phenol content of 520 mg AGE/g sample.

Keywords: microwave-assisted extraction (MAE), pegagan, ethanol, ANOVA, Folin-Ciocalteu.

REFERENCES

Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., & Rupasinghe, H. P. V. (2019). Response Surface Optimization for Recovery of Polyphenols and Carotenoids from Leaves of Centella asiatica Using an Ethanol-based Solvent System. Food Science and Nutrition, 7(2), 528–536. https://doi.org/10.1002/fsn3.832

Kristanti, Y., Widarta, I. W. R., & Permana, I. D. G. M. (2019). Pengaruh Waktu Ekstraksi dan Konsentrasi Etanol Menggunakan Microwave Assisted Extraction (MAE) Terhadap aktivitas Antioksidan Ekstraks Rambut Jagung (Zae mays L.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 8(1), 94–103. https://doi.org/10.24843/itepa.2019.v08.i01.p11

Rinawati, Pangesti, G. G., & Juliasih, N. L. G. R. (2020). Review : Green Analytical Chemistry: Pemanfaatan Supercritical Fluid Extraction (SFE) Dan Microwave Assisted Extraction (MAE) Sebagai Metode Ekstraksi Senyawa Diterpena Pada Minyak Biji Kopi Shangrai. Jurnal Analytical and Environmental Chemistry, 5(01), 24–33.

Sukmawati, Sudewi, S., & Pontoh, J. (2018). Optimasi dan Validasi Metode Analisis Dalam Penentuan Kandungan Total Flavonoid Pada Ekstrak Daun Gedi Hijau (Abelmoscus manihot L.) Yang Diukur Menggunakan Spektrofotometer UV-VIS. Pharmacon, 7(3), 32–41. https://doi.org/10.35799/pha.7.2018.20102

Sutardi, S. (2016). Kandungan Bahan Aktif Tanaman Pegagan dan Khasiatnya untuk Meningkatkan Sistem Imun Tubuh. Jurnal Litbang Pertanian, 35(3), 121–130. https://doi.org/10.21082/jp3.v35n3.2016.p121-130

Tahir, M., Muflihunna, A., & Syafrianti, S. (2017). PENENTUAN KADAR FENOLIK TOTAL EKSTRAK ETANOL DAUN NILAM (Pogostemon cablin Benth.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS. Jurnal Fitofarmaka Indonesia, 4(1), 215–218. https://doi.org/10.33096/jffi.v4i1.231

Yingngam, B., Chiangsom, A., & Brantner, A. (2020). Modeling and optimization of microwave-assisted extraction of pentacyclic triterpenes from Centella asiatica leaves using response surface methodology. Industrial Crops and Products,147(February), 112231. https://doi.org/10.1016/j.indcrop.2020.112231