Nur Aini Dwi Alfinaini
Department of Chemical Engineering, University of Jember, Indonesia 68121 

Boy Arief Fachri
Department of Chemical Engineering, University of Jember, Indonesia 68121 

Pratamai Shelli
Department of Chemical Engineering, University of Jember, Indonesia 68121 

Helda Wika Amini

Department of Chemical Engineering, University of Jember, Indonesia 68121 

Istiqomah Rahmawati
Department of Chemical Engineering, University of Jember, Indonesia 68121 

DOI: https://doi.org/10.19184/jobc.v3i1.278         

ABSTRACT
Coffea sp. is one of the largest plantation commodities in Indonesia, especially coffee leaves containing phenolic compounds. This research was conducted to extract phenolic compounds using the Ultrasound-Assisted Extraction method with ethanol solvent, which aims to determine the optimum operating conditions of extraction and the influence of variations in amplitude, time, and ratio of solvents used. The experimental design used Design Expert 13 software with the response surface method box-Behnken design. The research variables used were amplitude variations (50%, 60%, and 70%), time (10, 20, and 30 minutes), and solvent ratios (0.1, 0.15, and 0.2 g/mL). Based on our study, these parameters affect the total phenolic content. The model equation for the total phenolic content of coffee leaves obtained is Y = 0.1349 – 0.0016 A – 0.0505 B + 0.0010 C + 0.0018 AB + 0.0043 AC – 0.0018 BC – 0.0004 A2 + 0.0178 B2 – 0.0014 C2 (R2 = 0.9758) with the optimum total phenolic content located in the 17th running of 0.209 mg GAE/g under conditions of 20 minutes, the ratio of material to solvent is 0.2 g/mL, and an amplitude of 50%.

Keywords: Coffea sp., phenolic compound, ethanol, ultrasound-assisted extraction

REFERENCES

Alfinaini, N. A. D. A., Boy Arief Fachri, Pratamai Shelli, Helda Wika Amini, & Istiqomah Rahmawati. (2023). Extraction of Phenolic Active Compounds from Coffee Leaves (Coffea sp.) Using the Ultrasound Assisted Extraction Method and Total Phenol Analysis. Journal of Biobased Chemicals, 3(1), 25–39. https://doi.org/10.19184/jobc.v3i1.278

Amini, H. W., Pratiwi, W., Hartanto, G. P. ., Palupi, B., Fachri, B. ., Rizkiana, M. F., & Rahmawati, I. (2022). Ekstraksi Limbah Kulit Kopi Robusta Dari Desa Tanah Wulan Kecamatan Maesan Kabupaten Bondowoso Dengan Etil Asetat Serta Analisa Aktivitas Antioksidannya. E - Prosiding Kolokium Hasil Penelitian Dan Pengabdian Kepada Masyarakat, 87–92.

Andriani, M., Permana,  i D. G. M., & Widarta, I. W. (2019). Pengaruh Suhu dan Waktu Ekstraksi Daun Belimbing Wuluh (Averrhoa Bilimbil.) Terhadap Aktivitas Antioksidan Dengan Metode Ultrasonic Assisted Extraction (UAE). Jurnal Ilmu Dan Teknologi Pangan, 8(3), 330–340.

Ardianti, A., & Kusnadi, J. (2014). Ekstraksi Antibakteri Dari Daun Berenuk (Crescentia cujete Linn.) Menggunakan Metode Ultrasonik. Jurnal Pangan Dan Agroindustri, 2(2), 28–35.

Budiawan, L., Susilo, B., & Hendrawan, Y. (2014). Pembuatan Dan Karakterisasi Briket Bioarang Dengan Variasi Komposisi Kulit Kopi Preparation and characterization of bio charcoal briquettes from sawdust and coffee shell with variation of composition coffee shell. Pembuatan Dan Karakterisasi Briket Bioarang Dengan Variasi Komposisi Kulit Kopi. Jurnal Bioproses Komoditas Tropi. Vol. 2, No. 2. 2014. Hal: 152-160., 2(2), 152–160. http://webcache.googleusercontent.com/search?q=cache:TGTIz9W-ODUJ:jbkt.ub.ac.id/index.php/jbkt/article/view/151+&cd=1&hl=id&ct=clnk&client=firefox-b-abv

Campa, C., Mondolot, L., Rakotondravao, A., Bidel, L. P. R., Gargadennec, A., Couturon, E., La Fisca, P., Rakotomalala, J. J., Jay-Allemand, C., & Davis, A. P. (2012). A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Annals of Botany, 110(3), 595–613. https://doi.org/10.1093/aob/mcs119

Chen, S., Zhang, H., Yang, L., Zhang, S., & Jiang, H. (2023). Optimization of Ultrasonic-Assisted Extraction Conditions for Bioactive Components and Antioxidant Activity of Poria cocos (Schw.) Wolf by an RSM-ANN-GA Hybrid Approach. Foods, 12(3). https://doi.org/10.3390/foods12030619

Chen, X., Ding, J., Ji, D., He, S., & Ma, H. (2020). Optimization of ultrasonic-assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology. Journal of Food Science, 85(6), 1742–1751. https://doi.org/10.1111/1750-3841.15111

Dewi, R. P., Saputra, T. J., & Widodo, S. (2021). Studi Potensi Limbah Kulit Kopi Sebagai Sumber Energi Terbarukan Di Wilayah Jawa Tengah. Journal of Mechanical Engineering, 5(1). https://doi.org/10.31002/jom.v5i1.3946

Fachri, B. A., Abdilla, R. M., Rasrendra, C. B., & Heeres, H. J. (2015). Experimental and modelling studies on the uncatalysed thermal conversion of inulin to 5-hydroxymethylfurfural and levulinic acid. Sustainable Chemical Processes, 3(1), 1–11. https://doi.org/10.1186/s40508-015-0035-4

Fitriandi, P., Hardiani, H., & Mustika, C. (2019). Analisis sub sektor perkebunan Provinsi Jambi. E-Jurnal Perspektif Ekonomi Dan Pembangunan Daerah, 8(1), 15–30. https://doi.org/10.22437/pdpd.v8i1.5006

Guglielmetti, A., D’Ignoti, V., Ghirardello, D., Belviso, S., & Zeppa, G. (2017). Optimisation of ultrasound and microwave-assisted extraction of caffeoylquinic acids and caffeine from coffee silverskin using response surface methodology. Italian Journal of Food Science, 29(3), 409–423.

Hapsari, A. T., Kunarto, B., & Putri, A. S. (2021). Ekstraksi Kulit Kopi Robusta (Coffea canephora) Pada Berbagai Lama Waktu Ultrasound-Assisted Extraction Terhadap Antosianin dan stabilitasnya Selama Pemanasan. Jurnal Mahasiswa, Food Technology Agricultural Product, 3(2), 81–91.

Hartanti, A. I., Dewa, I., Mayun Permana, G., Kadek, G. A., & Puspawati, D. (2021). Itepa: Jurnal Ilmu dan Teknologi Pangan, Pengaruh Konsentrasi Etanol Pada Metode Ultrasonikasi Terhadap Aktivitas Antioksidan Ekstrak Daun Gonda (Sphenoclea zeylanica) Effect Of Ethanol Concentration In Ultrasonication Method On Antioxidant Activity Of Gonda Leaf Extract (Sphenoclea zeylanica). Itepa (Jurnal Ilmu Dan Teknologi Pangan), 10(2), 163–171.

Haslina, Nazir, N., Wahjuningsih, S. B., & Larasati, D. (2019). The influence of type of solvent and extraction temperature of corn silk extracts. International Journal on Advanced Science, Engineering and Information Technology, 9(3), 911–915. https://doi.org/10.18517/ijaseit.9.3.9037

Ibrahim, A. M., Sriherfyna, F. H., & Yunianta. (2015). Pengaruh Suhu dan Lama Waktu Ekstraksi Terhadap Sifat Kimia dan FIsik pada Pembuatan Minuman Sari JAhe Merah (Zingiber officinale var. Rubrum) dengan Kombinasi Penambahan Madu sebagai Pemanis. Jurnal Pangan Dan Agroindustri, 3(2), 530–541.

Kusnadi, J., Andayani, D. W., Zubaidah, E., & Arumingtyas, E. L. (2019). EKSTRAKSI SENYAWA BIOAKTIF CABAI RAWIT (CAPSICUM FRUTESCENS L.) MENGGUNAKAN METODE EKSTRAKSI GELOMBANG ULTRASONIK Extraction of Bioactive Compound in Chilli Pepper (Capsicum frutescens L.) with Ultrasonic Asissted Extraction Methods. Jurnal Teknologi Pertanian, 20(2), 79–84.

Kusumocahyo, S. P., Wijaya, S., Dewi, A. A. C., Rahmawati, D., & Widiputri, D. I. (2020). Optimization of the extraction process of coffee pulp as a source of antioxidant. IOP Conference Series: Earth and Environmental Science, 443(1). https://doi.org/10.1088/1755-1315/443/1/012052

Ladeska, V., Saudah, S., & Inggrid, R. (2022). Potensi Antioksidan, Kadar Fenolat dan Flavonoid Total Ranting Tetracera indica serta Uji Toksisitas terhadap sel RAW 264,7. Jurnal Sains Farmasi & Klinis, 9(2), 95. https://doi.org/10.25077/jsfk.9.2.95-104.2022

Lazuardina, B. A., Farah, D., Purba, W., Rusindiyanto, & Defri, I. (2022). Pemanfaatan Limbah Daun Kopi Sebagai Minuman Kesehatan di Desa Suberrejo, Jawa Timur. Jurnal Pengabdian Masyarakat Teknik Mesin, 2(1), 72–80.

Lestari, L. G. M., Antara, N. S., & Suwariani, N. P. (2021). Pengaruh Suhu Awal dan Waktu Infusi terhadap Aktivitas Antibakteri dan Antioksidan Ekstrak Minuman Herbal Daun Kopi Robusta. Jurnal Rekayasa Dan Manajemen Agroindustri, 9(1), 85. https://doi.org/10.24843/jrma.2021.v09.i01.p09

Mardwianta, B., Subarjo, A. H., Hermawan, S. D., & Husaini, M. (2020). Pengolahan Bioarang sebagai Pengganti Biogas untuk Mendukung Proses Roasting Pengolahan Kopi Arabica dan Ketahanan Energi. Angkasa Jurnal Ilmiah Bidang Teknologi, 12(1).

Marlina Kristina, C. V., Ari Yusasrini, N. L., & Yusa, N. M. (2022). Pengaruh Waktu Ekstraksi Dengan Menggunakan Metode Ultrasonic Assisted Extraction (UAE) Terhadap Aktivitas Antioksidan Ekstrak Daun Duwet (Syzygium cumini). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 11(1), 13. https://doi.org/10.24843/itepa.2022.v11.i01.p02

Noviyanti. (2016). Pengaruh kepolaran pelarut terhadap aktivitas antioksidan ekstrak etanol daun jambu brazil batu (Psidium guineense L.) dengan metode DPPH. Jurnal Farmako Bahari, 7(1), 29–35.

Pertiwi, M. G. (2018). Aplikasi Response Surface Methodology ( RSM ) untuk Meningkatkan Kuat Tekan Paving Block dengan Campuran Abu Ampas Tebu. Artikel Ilmiah Tugas Akhir, 1–15.

Putra, I. K. W., Puta, G. P. G., & Wrasiati, L. P. (2020). Pengaruh Perbandingan Bahan dengan Pelarut dan Waktu Maserasi terhadap Ekstrak Kulit Biji Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan The Effect Of Ratio Between Material And Solvent And Maceration Time On Cocoa Beans Husk Ekstract (Theobroma ca. Jurnal Rekayasa Dan Manajemen Agroindustri Vol., 8(2), 167–176.

Rahmawaty, F., & Sutanto, H. T. (2014). Penerapan Metode Permukaan Respon untuk Optimalisasi Proses Sealing pada Pengemasan Produk Makanan Jelly. Jurnal Ilmiah Matematika, 3(1), 1–6.

Ramadhani, S. F., Utama, M. J., & Ariani, A. (2023). Pembuatan Biobriket Dari Limbah Kopi Dan Sekam Padi Sebagai Bahan Bakar Alternatif. DISTILAT: Jurnal Teknologi Separasi, 7(2), 210–217. https://doi.org/10.33795/distilat.v7i2.224

Ristiana, D. (2017). Aktivitas Antioksidan Dan Kadar Fenol Berbagai Ekstrak Daun Kopi (Coffea Sp.): Potensi Aplikasi Bahan Alami Untuk Fortifikasi Pangan. Jurnal Aplikasi Teknologi Pangan, 6(2), 89–92. https://doi.org/10.17728/jatp.205

Riza Marjoni, M., & Devi Novita, A. (2015). Kandungan Total Fenol Dan Aktivitas Antioksidan Ekstrak Air Daun Kersen (Muntingia calabura L.) Total Content of Fenol and Antioxidant Activity of The Aqueous Extract of Cherry Leaf (Muntingia calabura L.). Jurnal Kedokteran Yarsi, 23(3), 187–196.

Rohmah, F. U., Rahmawati, A., Rizkiana, M. F., & Susanti, A. (2022). Optimization of Extraction of Bioactive Compound from Pegagan Leaves Using Ethanol Solvent With Microwave-Assisted Extraction Method (MAE). Journal of Biobased Chemicals, 2(1), 40–52. https://doi.org/10.19184/jobc.v2i1.119

Rondang Tambun, Harry P. Limbong, Christika Pinem, & Ester Manurung. (2017). Pengaruh Ukuran Partikel, Waktu Dan Suhu Pada Ekstraksi Fenol Dari Lengkuas Merah. Jurnal Teknik Kimia USU, 5(4), 53–56. https://doi.org/10.32734/jtk.v5i4.1555

Saadatian, M., & Asiaban, K. (2019). Effect of solvent, time and temperature on the some chemical properties of Salep tuber (Anacamptis Collina). Internatioanl Journal of Botany Studies, 4(4), 71–77. https://www.researchgate.net/publication/334524025_International_Journal_of_Botany_Studies_Effect_of_solvent_time_and_temperature_on_the_some_chemical_properties_of_Salep_tuber_Anacamptis_Collina

Sari, B. L., Triastinurmiatiningsih, T., & Haryani, T. S. (2020). Optimasi Metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Alga Coklat Padina australis. ALCHEMY Jurnal Penelitian Kimia, 16(1), 38. https://doi.org/10.20961/alchemy.16.1.34186.38-49

Saverus. (2019). No 主観的健康感を中心とした在宅高齢者における 健康関連指標に関する共分散構造分析Title. Jurnal Kajian Pendidikan Ekonomi Dan Ilmu Ekonomi, 2(1), 1–19. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865607390&partnerID=tZOtx3y1%0Ahttp://books.google.com/books?hl=en&lr=&id=2LIMMD9FVXkC&oi=fnd&pg=PR5&dq=Principles+of+Digital+Image+Processing+fundamental+techniques&ots=HjrHeuS_

Setiawan, E. A., Rahardian, D., & Siswanti. (2015). Pengaruh Penyaringan Daun Kopi Robusta (Coffea robusta) Terdahap Karakteristik Kimia dan Sensory Minuman Penyegar. Jurnal Teknosains Pangan, 4(2), 1–9. https://jurnal.uns.ac.id/teknosains-pangan/article/view/4678/4062

Setyawan, B., & Ulfa, R. (2019). Analisis mutu briket arang dari limbah biomassa campuran kulit kopi dan tempurung kelapa dengan perekat tepung tapioka. Edubiotik : Jurnal Pendidikan, Biologi Dan Terapan, 4(02), 110–120. https://doi.org/10.33503/ebio.v4i02.508

Suryanto, E., & Taroreh, M. R. I. (2020). ULTRASOUND-ASSISTED EXTRACTION ANTIOKSIDAN SERAT PANGAN DARI TONGKOL JAGUNG (Zea mays L.). Chemistry Progress, 12(2). https://doi.org/10.35799/cp.12.2.2019.27932

Syakfanaya, A. M., Saputri, F. C., & Mun’im, A. (2019). Simultaneously extraction of caffeine and chlorogenic acid from Coffea canephora bean using natural deep eutectic solvent-based ultrasonic assisted extraction. Pharmacognosy Journal, 11(2), 267–271. https://doi.org/10.5530/pj.2019.11.41

Tangguh, P., & Kusumocahyo, S. P. (2017). Extraction of coffee silverskin to convert waste into a source of antioxidant. AIP Conference Proceedings, 1803. https://doi.org/10.1063/1.4973156

Unzilatirrizqi D., Y. E. R., & Wadli. (2022). An alternative bio-briquettes energy of coffee grounds and onion waste combination. Jurnal Ilmu Lingkungan, 16(2), 141–149. https://doi.org/10.31258/jil.16.2.p.

Vermerris, W., & Nicholson, R. (2006). Phenolic compound biochemistry. Phenolic Compound Biochemistry, 1–276. https://doi.org/10.1007/978-1-4020-5164-7

Wibowo, H., Kunarto, B., & Larasati, D. (n.d.). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BIJI KELOR (Moringa oleifera) PADA BERBAGAI LAMA EKSTRAKSI BERBANTU GELOMBANG ULTRASONIK Antioxidant activity of moringa seed (Moringa oleifera) etanolic extract on ultrasonic-assissisted extraction long tiem.

Wijaya. (2022). Penetapan Kadar Air Simplisia Daun Kemangi (Ocimum Basilicum L.) Berdasarkan Perbedaan Metode Pengeringan. Jurnal Riset Kefarmasian Indonesia, 4(2), 185–199.

Yılmaz, T., & Tavman, S. (2017). Modeling and Optimization of Ultrasound Assisted Extraction Parameters using Response Surface Methodology for Water Soluble Polysaccharide Extraction from Hazelnut Skin. Journal of Food Processing and Preservation, 41(2). https://doi.org/10.1111/jfpp.12835

Yuliani, S. H., Sandrapitaloka, A. S., Restiana, F. R., Aji, P. D. T., Gani, M. R., & Riswanto, F. D. O. (2019). Effects of particle size, extraction time, and solvent on daidzein yield extracted from tempeh. Jurnal Farmasi Sains Dan Komunitas, 16(1), 44–49.

Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1), 1–26. https://doi.org/10.1186/s13020-018-0177-x